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Molecular cloning All plasmids were constructed using restriction enzyme digestion and ligation following standard protocols from New England Biolabs (NEB)

Processing of tissue samples and hemoglobin estimation Blood, 100 L of sample drawn by cardiac puncture under ether anesthesia (50 g/kg body weight) are centrifuged and washed twice with 5 mL of 0,9% NaCl

Helps with transforming carbohydrates into energy

One more thing: the water used in cosmetics is purified and deionized (it means that almost all of the mineral ions inside it is removed)