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Cells were then incubated at 37 C for the indicated time points in a mixture containing 1x Hanks balanced salt solution (HBSS, Gibco) or DMEM, each supplemented with 1 mM MgCl 2 , and 125 U Benzonase nuclease (Sigma/Pierce)

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While anyone could benefit from a shot of B12, weve found that the best candidates are people who have a B12 deficiency, are struggling to get all the vitamins through a vegan or vegetarian diet, someone who is older, or someone who has digestive or autoimmune issues

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