The cell suspensions were incubated with 5 L of FITC-conjugated Annexin V (V13242, Thermo) for 15 min at room temperature without exposure to light, followed by washing and resuspension in binding buffer
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The integral snorkels enable swimmers to keep their mouths closed, inhaling and exhaling air through their noses instead, while they are at, or just below, the surface of the water
Once it gets into the system, it starts shaping behavior in ways that feel subtle at first and then become harder to ignore
E-cigarettes are powered by lithium-ion batteries, which pose a fire risk if damaged, hence they are required to be carried within the plane cabin