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10 slides of each lung were examined by Olympus BX41 microscope and images were acquired by Olympus DP72 digital camera, followed by image analysis using ImageJ software (available from To quantify emphysema, alveolar enlargement and destruction were evaluated by the mean linear intercept (Lm) in 10 randomly selected fields per slide

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The total number of VAR models were summarized to compare participants descriptively without testing and should be viewed as exploratory analyses