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Chemical analysis To determine the nutrient contents in first flue-cured tobacco leaves, 1 kg leave sample was randomly taken in each treatment, in which the main veins were removed, and grinded and sieved through a 0.4 mm sieve before analyzing the chemical components ( N -methyl anabasine, -nicotyrine, anabasine, anatabine, isonicotenine and cotinine, were determined by gas chromatography-mass spectrometry method ( Soil organic C and total N were determined by the potassium dichromate volumetric method ( and NO) contents were measured by copperized cadmium reduction-diazotization coupling colorimetric method

The films script is loosely based on the Raymond Chandler novel The Big Sleep

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Campbell Washington said many those diseases could be linked to smoking