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Superoxide dismutase (SOD) activity was measured using the nitro blue tetrazolium (NBT) reduction method (Sadak et al., 2020), and peroxidase (POD) and catalase (CAT) activities were determined using the guaiacol method (Rcz et al., 2018) and ultraviolet spectrophotometry ( 2 O 2 ) content was determined using the titanium sulfate colorimetric method ( 2.4.1 Determination of plastid pigments Chlorophyll and carotenoids in tobacco leaves were measured using the ethanol-spectrophotometer method ( 2.5 Data analysis of physiological indicators The mean and standard deviation were calculated using IBM SPSS Statistics 27.0 software, and an independent samples t-test was used to analyze the significance of differences

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