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Study participants were divided into four different type 2 diabetes subtypes: Mild age-related diabetes (MARD) Mild obesity-related diabetes (MOD) Severe insulin-deficient diabetes (SIDD) Severe insulin-resistant diabetes (SIRD) Scientists also looked at the smoking status of participants, such as current, past, or nonsmoker

Stevens P, et al

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In each case, the microsomal suspension (1 mg protein) was incubated with 50 l smoke solution in a final volume of 20 l of 50 mM potassium phosphate buffer, pH 7.4 for 2 hours at 37 C