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Studies in mice investigating the effects of e-cigarettes on different brain regions have found that e-cigarette exposure during pregnancy can alter the transcriptome of the frontal cortex (1316 mg/mL nicotine), increase global DNA methylation within the brain (1316 mg/mL nicotine), and dysregulate gene expression in the hippocampus (13 mg/mL nicotine) [176]

The results from animal studies and in vitro experiments show that nicotine exposure results in -cell toxicity 100102

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