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Five microliters of each cDNA sample was added to a 20 l PCR mixture containing 12.5 l of the iQ SYBR Green supermix, 0.5 l of specific primers ACT1, SAP2, HWP1, and EAP1 (Invitrogen Life Technologies Inc., Burlington, ON, Canada), and 7 l of RNase/DNase-free water (MP Biomedicals, Solon, OH, USA)

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You'll also want to replace all filters in the house, including filters in the furnace, exhaust fans, and air conditioning units

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Hungry North Koreans are swooping in ahead of cleaning crews for large public events to collect the butts of filtered cigarettes, which they then sell for meager sums of cash, residents in the country told Radio Free Asia