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After washing three times for 15 min each with 0.3% Triton X-100 in PBS and three times for 1 min each with PBS, the optical clearing started by incubating the slices in 25% fructose for 4 h, continued in 50% fructose for 4 h, 70% fructose overnight, 100% fructose (100% wt/vol fructose, 0.5% 1-thioglycerol and 0.1 M phosphate buffer, pH 7.5) overnight, followed by a final overnight incubation in SeeDB solution (80.2% (wt/wt) fructose, 0.5% 1-thioglycerol and 0.1 M phosphate buffer) 63

How it works: MOTS-c activates AMPK, our bodys energy sensor, to theoretically mimic the effects of exercise at a cellular level

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Empirical entropic contributions in computational docking: Evaluation in APS reductase complexes