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Dual-luciferase assay HEK293T cells were transfected with 10 ng each of psiCHECK-2 construct along with 15 pmol of the miR-483-3p mimic or an identical amount of the control mimic with Lipofectamine 2000 (Invitrogen)

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Characterization of conserved Calcr -expressing cell populations The Glu4.2, Glut8.0 and Glu8.2 populations expressed Calcr across all three species, although Glu4.2 and Glu8.2 contained more Calcr -expressing cells than Glu8.0 (Fig