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The GST tagged protein is purified by passing the clarified lysate through prepacked columns

The bloodbrain barrier is dysregulated in COVID-19 and serves as a CNS entry route for SARS-CoV-2

This procedure involved removing the supernatant and resuspending the mitochondrial pellet in an isolation buffer of 250 mM sucrose, 0.5 mM EDTA, and 10 mM Tris-HCl to achieve a final protein concentration of 20-25 mg/ml [2]

This may be particularly beneficial for individuals exposed to environmental toxins or those leading high-stress lifestyles

The body naturally uses peptides to regulate blood pressure and protect vessel walls