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Sections were subsequently incubated in the cocktail of Alexa Fluor 488-conjugated Streptavidin (ThermoFisher, 1:500) and Alexa Fluor 555-conjugated donkey anti-rabbit IgG (ThermoFisher

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Where clinically indicated, we advocate that a formal open nail bed biopsy should be performed by an experienced clinician

If a smoker's melanosis is destroyed by excessive smoking, as in the hard palate of reverse smokers, who smoke with the glowing part of the cigarette inside the mouth for different reasons, a pale depigmented surface is first seen, indicating the loss of the protecting melanin

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