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The removal of obstructing tissue was first achieved by open prostatectomy in early 1900s [16] and considered as gold standard for the surgical treatment, but later replaced by TURP

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After centrifugation (1600 g, 4 C for 5 min), supernatant was diluted by two volumes of NET-2 buffer (50 mM Tris-HCl (pH=7.5), 150 mM NaCl, 0.05% NP-40, 1x protease and 1x phosphatase inhibitors) and pre-cleared by protein G Dynabeads at 4 C for 1 h

Concentration: 1% (10 mg/mL) Lidocaine Hydrochloride

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