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Following H 2 O 2 treatment, cells were collected by centrifugation, resuspended in serum-free medium containing 10 M DCFH-DA, incubated for 30 min at 37C, washed with PBS to remove unreacted dye, resuspended in PBS and was detected at 485 nm excitation and at 535 nm emission using flow cytometry

Healthy plant oils that are liquid at room temperature such as olive and avocado oils tend to be better tolerated than solid fats such as butter, lard or other saturated animal fats

Biodegradation 22:2530

Most patients see meaningful LDL reductions within 6-8 weeks as weight loss begins and metabolic changes activate

the primary phase begins within minutes and lasts for up to 60 min, after by a secondary phase that continues for up to 120 min after the meal [10]