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Materials and methods Animals Animal work in this manuscript was approved by the Experimental Animal Manage Committee of Changzhi Medical College (DW2024025)

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After adding 0.4 ml of 1 M sodium citrate buffer, pH 6.0, the mixtures were incubated at 37 C for 10 min with shaking at 125 rpm on a rotary shaker NR-3 (TAITEC) to facilitate release of bound sulfur as H 2 S gas from the aqueous phase

It supports natural pathways involved in comfort, nerve signaling, and immune modulation, in part through activation of PPAR- and interactions with the bodys endocannabinoid system