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What Is NAD+ and Why Does It Matter

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Potters, G., De Gara, L., Asard, H

To test the functionality of the sensors, we cultured transiently transfected RAW 264.7 Ms with or without oxLDL for 24 h, stimulated the cells with LPS for 6 h and performed imaging of the sensors

The supernatant from the hypotonic solution was combined with the supernatant from the hypertonic solution, centrifuged to remove debris, and dialyzed against 20LmM Tris, pH 7.5 and 150LmM NaCl (Tris-buffered saline, TBS) overnight at 4 C