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From what we know, though, it seems that if testosterone does have an influence, the effect is complex, not linear

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Upon activation, T cells undergo intrinsic metabolic reprogramming, engaging glycolysis and mitochondrial respiration to sustain effector responses ( Despite the frequent exposure of immune cells to subphysiological temperatures in peripheral tissues, it remains largely unknown how such cold environments influence T cell behavior

An iScript cDNA synthesis kit (Bio-Rad, Hercules, CA) was used to amplify 1 g of RNA per reaction in a MyCycler thermal cycler (Bio-Rad)