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Membranes were washed with PBS and fixed with ROTIHistofix 4% for 10 min at RT

Cardiovasc Diabetol 22(1):49 Russell-Jones D, Vaag A, Schmitz O, Sethi BK, Lalic N, Antic S et al (2009) Liraglutide vs insulin glargine and placebo in combination with metformin and sulfonylurea therapy in type 2 diabetes mellitus (LEAD-5 met + SU): a randomised controlled trial

mL and units are related in many U-100 syringe listings, but they do not mean the same thing

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Using a sterile syringe, draw the required volume of Bacteriostatic Water and inject it slowly down the inside wall of the peptide vial do not spray directly onto the lyophilised powder