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2.10 Cell lines and cell culture This study used the human epidermoid carcinoma cell line KB-3-1 as the drug-sensitive cell line, and its MRP1-overexpressing cell line KB/CV60 (Supplementary Figure S1A), which was maintained in the medium with 1 g/mL of cepharanthine and 60 ng/mL of vincristine (Supplementary Figure S1B) by transfecting recombinant pcDNA3.1/MRP7 plasmids and the parental human ovarian adenocarcinoma cell line SKOV3 ( 2
doi: 10.1136/gutjnl-2015-309147
Increases Energy Expenditure via Glucagon Receptor Activation What sets retatrutide apart is its activation of the glucagon receptor, which is associated with increased energy expenditure and fat utilization
doi: 10.1016/j.rceng.2022.09.003 15 LinharesBLMirandaEPCintraARRegesRTorresLO
On day 21, tumor progression was evaluated using bioluminescence imaging with the IVIS system (Caliper Life Sciences) following intraperitoneal injection of D-Luciferin potassium salt (3 mg/kg per mouse, administered 5 minutes prior to imaging, Beyotime, ST198) and anesthesia with 1% pentobarbital sodium (80 mg/kg per mouse)
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