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would be to regulate access of H 2 O 2 to the active site in such a manner that Compound 1 is only formed when a second molecule of H 2 O 2 is present to complete the reaction . [Dominguez, 2010] Gate opening would therefore be dependent on H 2 O 2 concentration at the accesses to the gate (molecular modelling shows that at 6M H 2 O 2 concentration, all the gates would be open, but that at ~300 mM H 2 O 2 concentration, only half the gates would be open), which could help explain the observation that catalase enzyme activity increases with H2O2 concentration [Diaz, 2001

Set the vial down and leave it undisturbed for 15 to 30 minutes