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The supernatant containing viral particles was harvested at 48 and 72 h after transfection, and was filtered through Millex-GP Filter Unit (0.45 m pore size, Millipore)

The findings demonstrate that morroniside alleviates neuropathic pain by activating GLP-1R and modulating spinal microglial function, leading to IL-10-mediated -endorphin production and subsequent activation of -opioid receptors [74]

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3.2 Opioids Although opioids such as morphine, remifentanil, heroin, and oxycodone largely share a common pharmacologic mechanism of action, namely, acting on opioid receptors, the ability of GLP-1R to regulate the behavioral responses to these opioids varies