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The metabolomic analysis was performed by following the Chemical Isotope Labeling (CIL) LC-MS protocol described in our previous report (81)

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CRISPR/Cas9 dropout screens Genome-wide CRISPR/Cas9 dropout screens were performed in Cas9-expressing TaC12 cells using a two-vector system, as well as small-scale screens in TaC12 and BoMac cells