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Biochem Mol Biol Educ

Science (1999) 284:13625

After washing with PBS thrice, the stained cells were analyzed using BD-Cytoflex flow cytometer (BD Biosciences)

They are produced by different types of cells and, once released in the body, induce specific reactions in adjacent cells (paracrine effect), in others far away (endocrine effect) or in those that have created them (autocrine effect)