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On the experimental front, the development of stem cell-derived human brain microvascular organoids and BBB-on-a-chip systems offers physiologically relevant in vitro models that recapitulate the cellular complexity and functional properties of the human BBB (244)

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This knowledge can now inform the design of ferroptosis modulators

coli , we reduced the reported disulfide bond between the active site cysteine C13 and GSH in the clarified cell lysate using dithiothreitol (DTT) and removed the reduced GSH with an extended on-column washing step during purification