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(2) Both BPC-157 and TB-500 are synthetic polypeptides, where TB-500 is composed of 43 amino acids, and BPC-157 is composed of 15 amino acids

RT-PCR An RNeasy kit (Qiagen, 74104) was used to extract total RNA from mice hippocampal tissue, and then the Transcriptor High Fidelity cDNA synthesis kit (Roche, 5081963001) was used to reverse transcribe the RNA into cDNA

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The authors also found increased alanine in the BPD group, which they attributed to increased cellular metabolism demands, due to an inflammatory process 47