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Other microRNAs of clinical significance are miR-95, miR-103, miR-106a, miR-151, miR-155, miR-182, miR-183, miR-194, miR-200a, miR-200c, miR-203, miR-205, miR-210, and miR-223

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Fluo-4 fluorescence intensity (excitation 480 nm, emission 520 nm) was measured as an indicator of calcium release in a multimode reader (Spark 20 M, TECAN)

In particular, autophagy level is downregulated in the early stage but upregulated in metastatic stage, which is under the control of histone deacetylase sirtuin 6 (SIRT6) via epigenetic modulation of insulin-like growth factor (IGF)-AKT pathway