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Protein bands were visualized using Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, Billerica, MA, USA) and a ChemiDoc MP Imaging System with Image Lab Touch Software Version 1.0 (Bio-Rad Laboratories, Hercules, CA, USA)

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For the immunostaining of cultured PSCs, cells were fixed in 4% PFA for 15 min followed by a 15-min incubation in 0.5% Triton-X100