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An automated high-throughput fluorescence in situ hybridization (FISH) assay platform for use in the identification and optimization of siRNA-based therapeutics

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As both H 2 O 2 and BCNU exposure led to excessive cellular GSSG accumulation, their impact on intracellular protein-GSSG adduct formation (protein S -glutathionylation) was subsequently examined in HepG2 cells by western blot analysis using a monoclonal GSSG antibody that specifically binds to GSSG-modified proteins