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The pellet was treated with permeabilization buffer and vortexed for 10 min at 4 with consistent mixing, followed by centrifugation at 15,000 g for 45 min at 4

Before propensity score matching, the semaglutide group included 127, 352, and 498 patients across the three BMI subgroups, respectively, while the control group included 11,277, 8,722, and 6,870 patients