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Specimen preparation is automated using the Cobas AmpliPrep instrument with amplification and detection automated using the Cobas TaqMan 48 analyser

The roles and intervention timing of calcium ion channel antagonists, hepcidin activators, antioxidants, and gene therapy are currently under further investigation

However, because they also slow digestion and promote feelings of fullness, many people who take them experience weight loss as a secondary effect

Acknowledgements This work was supported by grants from National Institute of Diabetes and Digestive and Kidney Diseases (R01DK121804) and National Institute of Food and Agriculture (67017-29252) Ethics declarations Competing interests The authors declare no competing interests

Patients need to understand that distinction