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The concentrations of protein extracts were determined by using the BCA Protein Assay Kit (Thermo Fisher Cat #23225), then protein samples were mixed with 3 loading buffer and loaded in equal amounts into the wells of 412% Bis-Tris protein gels (Bio-Rad) to transfer the proteins to PVDF membranes (Millipore)

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Physician Desk Reference, pdr.net

Macrophage polarization is subject to environmental cues

Glutamine as an essential amino acid for KRas-driven cancer cells