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The blots were incubated with primary antibodies against PGC-1 (Chemicon International, Temecula, CA) and AMP-activated protein kinase (AMPK) (Cell Signaling Technology, Beverly, MA), and the reaction products were visualized using a horseradish peroxidase-conjugated secondary antibody (Invitrogen, Carlsbad, CA) and enhanced chemiluminescence (Chemi-Lumi One Super, Nakarai Tesque, Kyoto, Japan) (Additional file 1: Figure S1)

Foreman KJ, Marquez N, Dolgert A, Fukutaki K, Fullman N, McGaughey M, et al

Injection sites rotate across the abdomen, outer thighs, and upper arms, avoiding the same spot on consecutive days

A ketogenic diet significantly enhances fatty acid metabolism and ketone body levels in rats with neural injury, markedly reducing the extent of myelin loss (Mu et al., 2022)