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The first dose is normally taken before the start of surgery, chemotherapy, or radiotherapy

For Safranin O/Fast Green staining, slides were stained with hematoxylin (Mayer, Sigma) for 5 min, 0.005% Fast Green (Thermo Fisher Scientific, Pittsburgh, PA, United States) for 1 min, rinsed with 1% acetic acid, and stained with 0.1% Safranin O (Millipore, Billerica, MA, United States) for 20 min

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doi: 10.3389/fendo.2018.00248 20 ReinstatlerLQiYPWilliamsonRS

Consultation with a healthcare provider should precede use