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The co-immunoprecipitation (Co-IP) assay was carried out using the Pierce Crosslink Magnetic IP/Co-IP Kit (88805, Thermo Fisher Scientific), where cell lysates were incubated with magnetic beads pre-bound to specific antibodies, as per the instructions provided by the manufacture

Together, these findings indicate enhanced KP engagement with a relative shift away from the astrocytic-KYNA branch toward the microglial-QA branch

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Adipose biology and obesity research robust reductions in adipocyte size and white-adipose mass in DIO mice without changes in food intake a phenotype distinct from any approved anti-obesity mechanism [2]