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The supernatants were filtered (0.2- m filter), transferred to glass HPLC vials, and kept at 80 C prior to performing a targeted metabolomic analysis consisting of 203 metabolite references at the UT Southwestern Medical Center Metabolomics Core Facility (Sciex QTRAP 6500 + mass spectrometer)

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These side effects can be permanent and are common in users with darker skin

48 This link to increased urine copper excretion raised the question whether liver damage per se could occur from copper toxicity