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Ultra-performance liquid chromatography-tandem mass spectrometry (UPLCMS/MS) UPLCMS/MS performed on a Waters Acquity UPLC-Xevo TQ-S UPLCMS/MS system equipped with an Acquity BEH HILIC (2.1 100 mm, 1.7 m) chromatographic column was employed to determine TMA, TMAO, choline, betaine, -butyrobetaine, betaine aldehyde, butyryl-carnitine, isovaleryl-carnitine, OH-isovaleryl-carnitine, stearoyl-carnitine, oleoyl-carnitine, linoleoyl-carnitine, myristoyl-carnitine, lauroyl-carnitine, and decanoyl-carnitine as described previously 31,41