Methods Yeast strains and plasmids The yeast strains used in this study include the wild-type Saccharomyces cerevisiae EG103 ( MAT leu2-3 112 his31 trp1-289a ura3-52 GAL + ) and the MnSOD-deficient yeast strain EG110 ( EG103 sod2 :: TRP1 ), kindly provided by Edith Gralla, University of California, Los Angeles and Valeria C
Creatine supplementation was performed by adding 2 % monohydrate creatine (Acros Organics, Fair Lawn, NJ, USA) to the control diet (AIN-93) ( Reference Reeves, Nielsen and Fahey 21 ) for the DCr group for 4 weeks
The MTHFR gene together with vitamin B9 and B12 plays an essential role in the methylation cycle , which is crucial for maintaining adequate homocysteine levels and obtaining methyl groups
You are over the age of 50 (when stomach acid naturally declines)
J Neurosci Res, 66(2), 266-71