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Protein lysates were harvested using Cell Lysis Buffer supplemented with Protease Inhibitor

By integrating continuously monitored vital signs and dynamically changing biomarker data (e.g., CRP, NLR every 12 hours), time-series analysis algorithms (e.g., recurrent neural networks, RNNs) can be used to build a dynamic, real-time updated risk warning system

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A small number of claudin genes (11) were expressed in ASG-10

The results revealed that patients receiving the combined therapy experienced significantly greater improvements compared to those in the control group