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Traditional methods have typically included high performance liquid chromatographic assays and are characterized by underestimation of GSH and overestimation of GSSG due to the autooxidative properties of GSH (Nuhu et al., 2020)

Minimal criteria for the histological diagnosis of definite NASH included the combined presence of grade 1 steatosis, hepatocyte ballooning, and lobular inflammation with or without fibrosis

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