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Subsequently, the samples were incubated with 3% H 2 O 2 to remove endogenous peroxidase activity and blocked with BDT (TBS containing 3% BSA and 10% goat serum) for 30 min, followed by overnight incubation with primary antibodies at 4 C

The discovery of glutathiones defense against oxidative stress caused researchers to investigate and discover that there is direct correlation between ageing and decreased intracellular concentrations of glutathione

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