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The column was eluted isocratically at a flow rate of 450 L/min for 1 minute at 80% mobile-phase A (10 mM NH 4 Ac/methanol/acetic acid 95:5:0.1, by volume), followed by a linear gradient to 80% mobile-phase B (methanol/acetic acid

My provider reviewed my file and approved treatment within 24 hours of the consultation