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Cells 11 , 2587 (2022)

The goal is observation , not evaluation

Its always best to draw from the sterile vial immediately before use

In addition, estrogens can inhibit the expression of BA metabolic enzymes, especially phase II enzymes (such as hydroxysteroid sulfotransferase 2a1, Sult2a1), which in turn decreases the metabolism of bile acids, leading to an increased levels of unconjugated and hydrophobic bile acids in hepatocyte, and a decrease in bile flow (Zamek-Gliszczynski et al., 2006