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Appl Microbiol Biotechnol 97:73997404

The measurable difference in final potency is small (under 3% in controlled studies), but for researchers prioritising maximum retention, cold-water reconstitution followed by immediate return to 28C storage is the conservative approach

Supporting this, previous NMR studies have shown that S129p significantly increases the hydrodynamic radius relative to Syn WT and alters its aggregation propensity, suggesting exposure of the hydrophobic core region 15

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