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cytochrome c (Cell Signaling, 12963S, clone 6H2.B4, lot 2, FI, 1:200, Hu)

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doi: 10.1111/j.1532-5415.2008.02051.x [DOI] [PubMed] [Google Scholar] 50

wherein X is Boc or Trt Fmoc-Ser(tBu)-Tyr(tBu)-Leu-Glu(OtBu)-Gly-OH (Fragment- 3) H-Gln(Trt)-Ala-Ala-Lys(Cl 8diacidmono-t -butyl -y-Glu(AEEA-AEEA)-OtBu)-Glu (OtBu)-Phe-Ile-Ala-Trp(Boc)-Leu-Val-Arg(Pbf)-Gly-Arg(Pbf)-Gly-OtBu (Fragment- 6) b) condensing H-Gln(Trt)-Ala-Ala-Lys(C 18diacidmono-t-butyl-y-Glu(AEEA-AEEA)- OtBu)-Glu(OtBu)-Phe-Ile-Ala-Trp(Boc)-Leu-Val-Arg(Pbf)-Gly-Arg(Pbf)-Gly-OtBu (Fragment-6) with Fmoc-Ser(tBu)-Tyr(tBu)-Leu-Glu(OtBu)-Gly-OH (Fragment-3) in presence of coupling agent and solvent in in-situ manner, followed by deprotection in presence of base to obtain H-Ser(tBu)-Tyr(tBu)-Leu-Glu(OtBu)-Gly-Gln(Trt)-Ala-Ala- Lys(C18diacidmono-t-butyl-Y-Glu(AEEA-AEEA)-OtBu)-Glu(OtBu)-Phe-Ile-Ala-Trp (B oc) -Leu- V al- Arg(pbf) -Gly- Arg (pbf) -Gly-OtBu