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Methods Cells and culture HEK293T cells obtained from the lab of Eitan Reuveny (Weizmann Institute of Science), were maintained in Dulbeccos Modified Eagle Medium (4.5 g/L d-glucose), supplemented with 10% fetal calf serum, 1% penicillinstreptomycin, 1% sodium pyruvate, 1% nonessential amino acids (Biological Industries), and MycoZap (Lonza cat

Sharma and Dubey 2005)

[51] Diagnosis [edit] According to one review: "At present, no 'gold standard' test exists for the diagnosis of vitamin B 12 deficiency and as a consequence the diagnosis requires consideration of both the clinical state of the patient and the results of investigations." [52] The vitamin deficiency is typically suspected when a routine complete blood count shows anemia with an elevated mean corpuscular volume (MCV)

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Mean age at diagnosis was 62 months (range: birth to 13 years)