A 20 l mix, containing 8 l of cDNA sample, 10 l of SYBR Green PCR Master Mix (Thermo Fisher Scientific), and 2 l of 10 mM forward primer and reverse primer mixture was used to performed Quantitative RT-PCR with a StepOne Plus Real-time PCR system (Applied Biosystems, Foster City, CA, United States)
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2.3.3 Asarone Asarone is a natural phenolic compound primarily derived from plants such as acorus calamus and asarum forbesii
ROS, generated by mitochondria, NADPH oxidase, or exogenous sources, disrupt Keap1, allowing Nrf2 to enter the nucleus and induce antioxidant genes for cytoprotection
Address: 13 Dien Bien Phu Street, Ward 3, Bac Lieu City Opening hours: 9.00 am to 6.00 pm Entrance fee: About $1 Nha Mat Tourist Area Nha Mat tourist area is a resort and ecotourism area combined with the largest entertainment area in Bac Lieu
