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The risk with aggressive fat loss including GLP-1 class compound-driven fat loss is lean mass loss

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Media and culture conditions Wild-type yeast strains were pre-cultured in 5 mL of YP20D medium containing 10 g/L yeast extract, 20 g/L Bacto Peptone, and 20 g/L glucose at 30 C and 250 rpm for 48 h

Evolutionarily, strict control of protein abundance is energetically costly

Acquisition of 1 H-NMR spectra and metabolic profiling The lyophilized metabolic extracts were suspended in 0.6 ml of deuterated water (Cambridge Isotope Laboratories Inc., Tewksbury, MA, USA) containing phosphate buffer (100 mM) and TSP (3-3-(trimethylsilyl)-2,2,3,3-tetradeuteropropionic acid or TMSP- d 4 , Cambridge Isotope Laboratories) (0.5 mM) and inserted into a 5 mm NMR tube for immediate acquisition