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Recombinant human procathepsin B (R&D systems) was activated to mature cathepsin B by incubation at 37 C for 20 min in activation buffer (20 mM sodium acetate pH 5.5, 1 mM EDTA, 5 mM DTT and 100 mM NaCl)

While some ASM participants may be associated with a Shared Savings Program ACO, we do not believe that it would be appropriate at this time to include Shared Savings Program metrics as part of the performance assessment for determining payment adjustments under ASM

NAD is important because its known to regulate mitochondrial signaling

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