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When applicable, cells were transfected 24 h later as described above

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RNA from the control and E2/LPS treated cells were analyzed by RT-qPCR for the expression of various cytokine (IL6, IL-1), IDO1, and SR-BI expression

For example, the class III GRXS3/4/5/8 proteins function downstream of cytokinins in Arabidopsis to negatively regulate primary root growth in response to nitrate (Patterson et al

While direct studies on iodine excess and reproductive immunology remain limited, further research is needed to clarify these potential mechanisms